Main container

Dr. Dirignani Irene

CV

Date of Birth
17/07/1996
Qualification

Biotechnologist

Current assignment

PhD student

Title of qualification awarded

2021: Master's Degree in Medical biotechnologies (final grade: 110/110 cum laude), University of Padua

Other qualifications awarded
  • 2023: II level Master in omics data analysis (final grade: Excellent), University of Padua  
  • 2018: Bachelor's Degree in Biotechnology (final grade: 108/110), University of Padua
  • 2022: Qualification to practice the profession of Biologist - section A
Positions held
  • November 2023 – present: PhD student (Course of Oncology, Hematology and Pathology Unibo) at the Experimental Oncology laboratory of the Rizzoli Orthopedic Institute, Bologna. 
  • December 2021 – September 2023: Fellow at the Immunology Research Group, National Institute of Molecular Genetics (INGM) 
  • May 2021 – September 2021: Post-graduation Erasmus+ trainee at the Max Delbrück Center for Molecular Medicine (Berlin) 
  • March 2020 – March 2021: master's thesis internship at the Ludwig Cancer Research Institute (Lausanne) 
  • March - July 2018: three-year degree thesis internship at the laboratory of Professor Brini, department of Biology
Languages

Italian: netive speaker
Inglese: excellent (C1) 
Francese: scholastic

Technologies
  • Isolation of PBMCs from buffy coats and tumor infiltrates using ficoll
  • Isolation of immune subpopulations (e.g., CD8+, CD4+ T lymphocytes, CD14+ monocytes) via MACS 
  • Isolation of immune subpopulations via FACS
  • Flow cytometry (intra and extracellular staining)
  • Cell cultures in suspension (CD8+, CD4+ T lymphocytes) and adhesion (HEK293, macrophages); co-culture of CD8+ T lymphocytes with APC 
  • Generation of CD8 T lymphocyte clones in Terasaki plates
  • ELISpot 
  • Isolation and characterization of extracellular vesicles 
  • Molecular Biology Techniques: nucleic acid extraction and quantification (Nanodrop, Qubit); PCR; Real Time PCR; Reverse transcription; protein extraction; Western Blotting; 
  • 3D cultures in matrigel of colon and lung organoids and tumors 
  • Softwares: Adobe Acrobat / Microsoft Office (Microsoft) / GraphPad Prism / Snapgene viewer / Image lab software (Bio Rad) / BD FACSDiva™ & FlowJo Software / Adobe Photoshop / ImageJ (Fiji) / QuantStudioTM Design & Analysis Software / R - Programming
Scientific activities

Study and characterization of the immune infiltrate in different tumors with the aim of identifying new therapeutic approaches